Not known Factual Statements About flow cytometers
Not known Factual Statements About flow cytometers
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Details-enriched characterization of mobile populations. Morphological info from visuals can insert on the richness of flow cytometry information. For instance, the determine exhibits an usually common apoptosis assay working with Annexin V and PI, including cell imaging to characterize cells in Each and every inhabitants to expose morphologically distinct features. These insights couldn't are already received from multiplex staining by yourself.
As samples are acquired on the Attune CytPix Flow Cytometer, the substantial-speed brightfield digital camera captures and retailers pictures of detected activities at a charge up to six,000 visuals per second, dependant upon the flow fee and picture dimension. For higher adaptability, Attune Cytometric Software program lets you alter the picture capture frequency as required and select a particular gate for picture seize, if required.
(B) Uncooked 264.7 macrophages are frequently annotated inaccurately with regard to particle count because of their complexity and noncircular borders. The quantity of singlets accessible for analysis will increase as soon as the consumer trains and employs a more exact product.
The award-profitable Invitrogen Attune CytPix Flow Cytometer brings together acoustic concentrating which has a large-velocity brightfield digicam for simultaneous superior throughput flow cytometry and significant resolution brightfield imaging.
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Below, a qualified consumer has gated singlets confidently. Right after assessing the handbook singlet gate, the CytPix image-derived parameter ParticleCount reveals this gate is made up of much more than four% aggregates.
Pair your Attune flow cytometer with an autosampler for increased workflow effectiveness, and add robotic integration for maximum throughput.
Constant graphic high-quality even at superior throughput Acoustic focusing plus a large-speed digital camera Incorporate to graphic these T cells continuously at small or superior flow charges. Conveniently modify concentrate and digicam settings to fulfill experimental prerequisites.
Early signs of this contamination experienced in the beginning been dismissed as debris. Backgating to discover new populations. Backgating imaged cells about the Attune CytPix Flow Cytometer also allows you to use morphological attributes to find out interesting subpopulations that would not be obvious from flow cytometry facts by itself.
Customers can watch function visuals in genuine-time to substantiate sample high-quality, enhance protocols, and document the morphology of each and every cell populace discovered for Assessment. Imaging seize also Added benefits from acoustic focusing, which really helps to place the cells to ensure a sharp, centered picture is acquired.
Sound waves permit exact mobile alignment, leading to exceptional facts high quality and ~10X speedier sample flow fees.
We Beforehand shown the power of imaging Vehicle-T/Ramos cell interactions. Permit’s evaluate just the populace of biggest fascination, the double favourable functions, to learn more. We could now use extended morphological parameters (right here, circularity and skewness of depth) to additional examine the functions of such populations and refine gating on these activities, growing details robustness.
Leverage the strength of Highly developed technological know-how and innovation to accelerate your discoveries. Belief within the Attune Flow Cytometer loved ones to deliver the reliability, speed, and precision that set your function aside.
Adaptable payment approach from Agilent Economic Solutions allow you to obtain the critical instrumentation you require for fast task, or to grow your lab.
At the guts of each Attune Flow Cytometer lies our innovative acoustic concentrating technology, which utilizes seem waves to meticulously align cells inside the flow cell. This ground breaking strategy surpasses common hydrodynamic methods, resulting in more quickly processing speeds, Excellent knowledge good quality, and increased sample throughput.
The Attune Xenith cytometer can be a large-parameter spectral analyzer, delivering each spectral unmixing and traditional flow capabilities for in depth and versatile cell Examination.
Agilent Price Assure ensures you at least 10 years of instrument use within the date you purchase. If not, We're going to credit history you Together with the residual price of the system towards an upgraded more info model.
Listed here, we present that by gating methods working with impression-primarily based quantitative parameters can distinguish interacting cells from coincident situations to a lot more correctly evaluate interacting cells.